ArticleBiz.com :: Free article content
Authors: Maximum article exposure. Publishers: Reprintable article content.
BROWSE ARTICLES
ArticleBiz.com Home
Featured Articles
Recently Added Articles
Most Viewed Articles
Article Comments
Advanced Article Search
AUTHORS
Submit Article
Check Article Status
Author TOS
PUBLISHERS
RSS Article Feeds
Terms of Service

When to (and not to) Use Ultrasonicator Systems?
Home Computers & Technology Technology
By: Paresh Shah Email Article
Word Count: 473 Digg it | Del.icio.us it | Google it | StumbleUpon it

  

Ultrasonic probe sonicators are versatile systems that find applications in many areas. Their applications range from emulsion preparation, dispersion, and protein extraction, to cosmetics manufacturing, tissue preparation, and degassing of liquids, amongst others. While different sonication systems are used for different applications, every system consists of three elements: a power generator that supplies input AC supply; a transducer (featuring piezoelectric converter) which converts electrical energy coming from the generator into high-frequency mechanical vibrations; and a probe or a horn which amplifies these vibrations by expanding and contracting longitudinally whilst transmitting them down as ultrasonic waves into the sample. The intensity of vibrations is highest at the tip of the probe thereby causing cavitation and leading to implosion of microscopic bubbles. This releases tremendous energy that can be used to process the sample.

Modern systems allow practitioners to control the tipís amplitude according to the sample being processed. The ultrasonicator systems also allow the user to set the sample processing in pulse mode or use temperature-monitoring mechanism to maintain the integrity of samples. However, there are applications when ultrasonic probe sonicators are not a suitable choice to process the sample. Some general parameters to assess whether ultrasonic homogenizers are suitable for a particular application or not include:

1. Character of your sample

Ultrasonic probe sonicators produce great results when the sample processed is typically liquid or small solid particles in a liquid in need of uniform homogenization. As a result, ideal applications include cell disruption, nanoparticle dispersion, and DNA extraction, to name a few. On the other hand, if the sample contains more of solids than the liquid, using sonicator systems may produce unsatisfactory results. Ultrasonicator systems may not be the best option for directly processing large tough tissues.

2. Heat sensitivity of your sample

Heat sensitivity of the sample is an important parameter to decide whether or not to go with ultrasonic processing. Since ultrasonic homogenizers generate large amount of heat, processing samples that are highly sensitive to heat may be inappropriate. Even if in some cases where sonicators are used, care should be taken to use a pulse mode or put together a recirculation loop setup comprising a sonicator, ultrasonic reactor, and a cooling jacket. As a result, since assays comprising RNA are heat-sensitive, using ultrasonication to process them is inappropriate.

3. Processing effect desired on your sample

Some applications require a shearing effect on the sample whilst others require a sample to be cut or tore apart. Ultrasonication systems form an ideal choice for the former category of applications, such as DNA shearing. Alternatively, if you need to tear apart your samples, probe sonicators donít form the ideal choice. Applications that fall into this category include processing tough animal tissues and cells.

Paresh Shah is a Director at Life-Care Equipments Pvt. Ltd., a leading manufacturer and distributor of a wide range of ultrasonic systems, including ultrasonicator units, bench top ultrasonic cleaners, ultrasonic atomizers, ultrasonic bath, and related accessories, such as ultrasonic reactor

Article Source:
http://www.articlebiz.com/article/1051632105-1-when-to-and-not-to-use-ultrasonicator-systems/

This article has been viewed 1953 times.

Rate Article
Rating: 0 / 5 stars - 0 vote(s).

Article Comments
There are no comments for this article.

Leave A Reply
 Your Name
 Your Email Address [will not be published]
 Your Website [optional]
 What is nine + four? [tell us you're human]
Notify me of followup comments via email


Related Articles


Copyright © 2020 by ArticleBiz.com. All rights reserved.

Terms of Service | Privacy Policy | Contact Us | Submit Article | Editorial